New York City (First and only known appearance).

New York (First and only known appearance).United States of America (First and only known appearance).Earth (First and only known appearance).Earth-TRN1003 (First and only known appearance).Hunter Killer Robots (Spiders) (First and only known appearance).Hunter Killers (First and only known appearance).Super Mech (First and only known appearance).Super Soldier Robots (First and only known appearance).Green Goblin (Norman Osborn) (Mentioned) (Deceased).Antower (First and only known appearance) Oscorp Technologies (First and only known appearance).Bonesaw McGraw (First and only known appearance).Green Goblin (Mercenary) (First and only known appearance).Kraven the Hunter (Sergei Kravinoff) (First and only known appearance) (Xbox only).Scorpion (MacDonald Gargan) (First and only known appearance).Shocker (Herman Schultz) (First and only known appearance).Spike (First and only known appearance).Mary Jane Watson (First and only known appearance).Green Goblin (Harry Osborn) (First and only known appearance).Human performance enhancers (First appearance).Shocker's Vibro-Shock Gauntlets (First appearance).Bronx Zoo (First appearance) (Xbox only).Vulture's lair, a Lower East Side clock tower (First appearance).Sony Plaza, 550 Madison Avenue (First appearance).Subway and sewer tunnels (First appearance).Grand Central Station (First appearance).Daily Bugle Building (First appearance) (Xbox and GBA only).United States of America (First appearance).Hunter Killer Robots (Spiders) (First appearance).

Super Soldier Robots (First appearance).Mendel Stromm (First and only known appearance dies).Bonesaw McGraw (First and only known appearance) (Not in GBA version).Green Goblin (Norman Osborn) (First and only known appearance dies).Kraven the Hunter (Sergei Kravinoff) (First appearance) (Xbox and GBA only).Scorpion (MacDonald Gargan) (First appearance).Vulture (Adrian Toomes) (First and only known appearance).

Shocker (Herman Schultz) (First appearance).

Spike (First and only known appearance dies).

Skulls (First and only known appearance).

Spider-Man (Peter Parker) (First appearance).

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